TIGS has a state-of-the-art greenhouse facility for growing transgenic and non-transgenic plants under controlled conditions. The greenhouse is carefully designed to avoid any unintentional transmission of recombinant or synthetic nucleic acid molecules through plant pollen and to avoid any escape and establishment of genetically engineered (GE) plants into the natural environment.
- State-of-the-art greenhouse facility for growing transgenic and non-transgenic plants under controlled conditions.
- Standard Operating Procedure (SOP) to conduct experiments on transgenic and non-transgenic plants in controlled conditions is designed as per the DBT guidelines.
- The initial screening of genetically engineered events takes place in the greenhouse after plant transformation and regeneration of whole plants in vitro.
- Greenhouse is also equipped with pollination chamber to perform crosses between selected GE plants and wild-type parent controls.
- The pollination chamber is designed to generate heat and humidity required to perform crossing experiments.
- We maintain different varieties of rice, finger millet and pigeonpea all year long in TIGS greenhouse to make available embryos for tissue culture and transformation experiments and pollen for backcross breeding.
- Currently we are evaluating genome edited rice lines and EMS mutagenized pointed gourd lines in TIGS greenhouse facility.
The primary transformants and their derivatives are usually grown for early trait evaluation and event screening purposes. We have proper screening and labelling procedures in place for the accurate identification of plants which is very critical to maintaining plant product integrity during research activities in containment facilities.
Green house at TIGS
Modified rice plant lines
Modified rice plant lines
Wild type finger millet plants